Enter X and Y
X in the first column, Y to the right; replicates side by side.
Paste X and Y values (with replicates if you have them) to get the slope and intercept with standard errors and 95% confidence intervals, R², Sy.x and whether the slope differs from zero.
Read unknown samples off a standard curve, or try a quadratic or cubic and compare the fits.
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X in the first column, Y to the right; replicates side by side.
The straight line is fitted at once, with a confidence band. Choose a polynomial, a line through the origin or weighting if needed.
Type absorbances to interpolate concentrations, copy the table, and save the graph.
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The fraction of the variation in Y explained by the line. It does not say whether a straight line is the right model: check the residuals and the runs test.
Choose “Compare with” to test whether a quadratic fits significantly better (F test and AICc).
Yes: choose “Line through the origin” as the equation, or fix YIntercept at 0 under Constraints.
No. The regression runs in your browser.
Slope ± SE · Standard curves · No upload